The 1941 Colorado Buffaloes football team was an American football team that represented the University of Colorado as a member of the Mountain States Conference (MSC) during the 1941 college football season.

Led by first-year head coach James J. Yeager, the Buffaloes compiled an overall record of 3–4–1 with a mark of 3–2–1 in conference play, tying for fourth place in the MSC.

Colorado was outscored by a total of 161 to 97 on the season.

116 (out of 681 teams) in the final rankings under the Litkenhous Difference by Score System for 1941.

[10][11][12] The following Buffalo was selected in the 1942 NFL draft following the season.