The Dutch Eerste Divisie in the 1960–61 season was contested by 36 teams, divided in two groups.
FC Volendam and Blauw-Wit Amsterdam won the championship.
Promoted from the 1959–60 Tweede Divisie: Relegated from the 1959–60 Eredivisie: Entered from the B-group: Promoted from the 1959–60 Tweede Divisie: Relegated from the 1959–60 Eredivisie: Entered from the A-group:
DHC moved to Eerste Divisie A next season.
VV Helmond moved to Eerste Divisie A next season.