Each region of Ukraine delegated one team to the competition.
The winner of the competition FC Enerhiya Nova Kakhovka was not promoted to the 1965 Ukrainian Class B (Soviet Class B, III tier).
Instead of them, to the Class B were promoted the 2nd place runner-up Shakhtar Krasnyi Luch.
In total 26 participants took place in this season competition.
Many former participants (10) of the 1959 Football Championship of the Ukrainian SSR joined this season competition.