The 1965 Canada Cup took place 30 September – 3 October at the Real Sociedad Hípica Española Club de Campo in Madrid, Spain.

The South African team of Harold Henning and Gary Player won by eight strokes over the Spanish team of Ángel Miguel and Ramón Sota.

The individual competition was won by Gary Player, who finished two shots ahead of Jack Nicklaus.

The Ryder Cup was played the following week and the team had a prior engagement to play in the Honda Foursomes Tournament which was contested at the same time as the Canada Cup.

Team Peter Thomson of Australia withdrew during the final round.