The 1990 NCAA Division I softball tournament was the ninth annual tournament to determine the national champion of NCAA women's collegiate softball.
Held during May 1990, twenty Division I college softball teams contested the championship.
The tournament featured eight regionals of either two or three teams, each in a double elimination format.
The 1990 Women's College World Series was held in Oklahoma City, Oklahoma from May 23 through May 28 and marked the conclusion of the 1990 NCAA Division I softball season.
For the third consecutive year, UCLA won the championship by defeating Fresno State 2–0 in the final game.