The 1994 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 1994 NCAA Division I-AA football season.
Led by Rick Rhoades in his second and final season as head coach, the Colonels compiled an overall record of 5–6 with mark of 1–5 in conference play, placing in a three-way tie for fifth in the Southland.
Nicholls State played home games at John L. Guidry Stadium in Thibodaux, Louisiana.
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