[2][3] The team competes in the NCAA Division I Football Championship Subdivision (FCS) and are members of the Southland Conference.

The team plays its home games at the 10,500 seat Manning Field at John L. Guidry Stadium in Thibodaux, Louisiana.

The stadium is named in honor of former state representative John L. Guidry who was instrumental in the establishment of Francis T. Nicholls Junior College.

The stadium features a three-level press box on the west side with a photo deck located on the roof.

Members use the club room as a hospitality area before home football games as well as for various university functions throughout the year.

On June 12, 2019, Nicholls announced a new $6.5 million, 20,000-square-foot football operations center will be built in the south end zone along with an expansion and renovation of the Frank L. Barker Athletic Building.

[11] The team meeting room will also function as a gameday club seating area with a large window overlooking the field.

The football rivalry game with Northwestern State is played annually with the winner being awarded the NSU Trophy.

"Voice of the Colonels" Jack Benjamin will provide play-by-play for the broadcasts and will be joined in the booth by Ronnie Rantz.