The show's current cast members are Kim Jong-min, Moon Se-yoon, DinDin, Lee Joon, Jo Se-ho, and Yoo Seon-ho.

Along with The Return of Superman, the show's third season helped revive and maintain KBS2 Happy Sunday ratings, beating out competition from other major networks since its first episode.

[6] Lee Yong-jin joined the cast as 2 Days & 1 Night's first ever intern in December 2018 after appearing as a guest.

[7] Jung Joon-young left the show on September 29, 2016 due to his scandal regarding a "sexual offence" charged by his former girlfriend, of which he was later cleared.

[8] On January 7, 2017, a production member confirmed that Joon-young will return to the show, already having recorded an episode the previous day.

Jung Joon-young was permanently removed on March 12, 2019 by the production team due to his involvement in the Burning Sun scandal concerning non-consensual filming during sex and covert distribution of said illegal videos.

[11] The chatroom exposé also affected members Cha Tae-hyun and Kim Jun-ho with allegations of golf gambling in large amounts, resulting in both of them quitting all their television programs, including 2 Days & 1 Night, with immediate effect.

[17] On October 20, 2021, both KBS and the production team confirmed that Kim Seon-ho has been removed as a permanent cast member following controversies regarding his personal life.

[23] On November 28, 2022, Yoo Seon-ho was confirmed to be the newest cast member,[24] with his first episode airing on December 11, 2022.

On June 9, 2024, Yeon Jung-hoon and Na In-woo announced their departure from the show,[25] with their last episode broadcast on July 21.

Season 2 aired from June 3 to September 16, 2009 with two members from Season 1, Kim Sook and Kim E-Z, and new members, actress and singer Yoo Chae-yeong, movie actress Seo Young, gagwoman Kwon Jin-young, and Kang Ye-bin.

The concept of 1 Night 2 Days is traveling on a trip with families and friends, while Infinite Challenge is a challenge-based variety program with 7 men competing in games.

In 2004, all 3 major broadcasting stations, KBS, MBC and SBS, agreed to prohibit smoking scenes being aired before midnight.

The scene was a hidden prank of Kang Ho-dong and Kim C getting into an argument to fool the new director who had joined the show.

[47] It was announced that on March 9, 2010, the cast and crew of 1 Night 2 Days will take a trip to Antarctica to visit the King Sejong Station.

The production crew had bought brand new HD cameras, lighting equipment and microphones to film the beauty of Antarctica and bring the best resolution to viewers.

The title of the song is called "Let's Go Paradise" and is sung by Gil Hak-mi (a contestant on Mnet's talent show Superstar K 1) and Han Bora (from AB Avenue).

[56] The 2015 KBS2 television series The Producers, written by Park Ji-eun and directed by Seo Soo-min, which depicts the lives of the employees of the KBS variety department, is centered around the (fictional) production staff of 2 Days & 1 Night.

Unlike the original program, however, the 2 Days & 1 Night depicted in the drama had both male and female cast members and was already in its fifth season.

[57][58] Several celebrities also made cameo appearances in the drama as (fictional) cast members from various seasons of the show, including Youn Yuh-jung, Geum Bo-ra, Hwang Shin-hye, Hyun Young, You Hee-yeol, Jun Hyun-moo, Yoon Jong-shin, Sandara Park, Kang Seung-yoon, Minwoo, Kim Min-jae and Jisoo.

At KBS Entertainment Awards, cast members of 2 Days & 1 Night won the Grand Prize in 2008, 2009, 2011, 2016 & 2021 and the show became Viewers' Choice Best Program in 2015, 2016, 2018, 2020 & 2021.