Lee Ik-jun is an assistant professor of general surgery specializing in liver transplants and a single father to his only son Woo-joo (Kim Joon) after getting divorced from his absent wife.

A devout Catholic, Ahn Jeong-won is the son of the founder of the hospital and an assistant professor of pediatric surgery, cherished by his patients for his caring and sensible personality.

Kim Jun-wan is an associate professor and later head of the cardiothoracic surgery department at Yulje, admired and feared by his subordinates due to his exceptional surgical skills and his cold demeanor.

Yang Seok-hyeong is an assistant professor of obstetrics and gynaecology perceived as aloof and sometimes detached due to his introverted personality, but he is in reality attentive and understanding toward his patients.

The only woman in the group of friends, Chae Song-hwa, is an associate professor of neurosurgery, considered as the perfect doctor by her colleagues and patients alike thanks to her brilliant career, accommodating disposition and bright personality.

In January 2019, it was reported that Shin Won-ho would direct a medical television series and that the production team was in the early stages of casting actors.

[64] The Hospital Playlist original soundtrack produced by Ma Joo-hee was released on June 4, 2020, by Studio MaumC, Egg Is Coming and Stone Music Entertainment.

[89] During an interview with The Korea Herald, film critic Yun Suk-jin praised Hospital Playlist for emphasizing the human aspect of the profession rather than the life-and-death situations, noting that the series "did away with the cliched power conflict, and focused on the doctors' sincerity in their work and their relationship with the patients.

[92] Joel Keller of Decider gave a mixed review, saying that the series "is a bit of a strange amalgamation of different genres, but the cast makes it work.

On September 20, 2023, it was announced that Shin and Lee would create a spin-off television series titled Resident Playbook, set to be broadcast on tvN in the first half of 2024.

Starring Go Youn-jung as a first-year obstetrics and gynecology resident, the series will be directed by Lee Min-soo and written by Kim Song-hee, who had worked as an assistant writer on both Reply 1988 and Hospital Playlist.