It was mustered in under the command of Colonel Frank Lane Wolford.
The regiment was attached to Thomas' Command, Camp Dick Robinson, Kentucky, to December 1861.
(5 companies attached to Garfield's 18th Brigade, Army of the Ohio, December 1861 to March 1862.)
1st Brigade, Cavalry Division, Army of the Ohio, to November 1862.
Post Gallatin, Tennessee, Department of the Cumberland, to April 1863.
District of Central Kentucky, Department of the Ohio, to June 1863.
1st Brigade, 1st Division, XXIII Corps, Army of the Ohio, to August 1863.
Independent Cavalry Brigade, XXIII Corps, to November 1863.
1st Brigade, 1st Division, Cavalry Corps, Army of the Ohio, to May 1864.
Independent Brigade, Cavalry Division, XXIII Corps, to August 1864.
4th Brigade, 1st Division, District of Kentucky, Department of the Ohio, to December 1864.
Camp Nelson, Military District of Kentucky, to September 1865.
The 1st Kentucky Cavalry mustered out of service at Camp Nelson on September 20, 1865.
(5 companies sent to Prestonsburg, Kentucky, December 10 and Join Garfield.
Garfield's operations against Humphrey Marshall December 23, 1861 to January 20, 1862.
Near Logan's Cross Roads, Mill Springs, on Fishing Creek, January 19–20, 1862.
Big Creek Gap and Jacksboro March 14 (detachment).
March to Louisville, Kentucky, in pursuit of Bragg August 21-September 26.
Sterling, Kentucky, February 17–26, and duty there reorganizing until April.
Operations on line of Pumpkin Vine Creek and battles about Dallas, New Hope Church, and Allatoona Hills May 25-June 5.
Veterans and recruits consolidated to a battalion of 3 companies and on duty at various points in Kentucky, operating against guerrillas and quieting country, until September 1865.