The regiment served out its term of service within the continental United States, and did not see action during the war.

[1] The 1st Wisconsin was mustered into service on May 14, 1898, at Camp Harvey in Milwaukee, Wisconsin, with a strength of fifty officers and 976 enlisted men.

The regiment moved to Camp Cuba Libre in Jacksonville, Florida, and was assigned to the 2nd Brigade, 2nd Division of the 7th Army Corps commanded by Fitzhugh Lee.

The corps was being trained for an intended assault on Havana, Cuba, when an armistice ended the fighting on August 12, 1898, though the war would officially continue until the signing of the Treaty of Paris on December 10, 1898.

At the time of mustering out, the unit consisted of forty-nine officers and 1,224 enlisted men.