The 2000-01 Division 1 season was the 36th of the competition of the first-tier football in Senegal.

The tournament was organized by the Senegalese Football Federation.

[1] The season began on 11 November and finished on 24 June 2000.

ASC Jeanne d'Arc won the eighth title and participated in the 2002 CAF Champions League the following year.

The league was contested by 14 teams with ASC Jeanne d'Arc winning the championship.