The 2000–01 season of the NOFV-Oberliga was the seventh season of the league at tier four (IV) of the German football league system.
The NOFV-Oberliga was split into two divisions, NOFV-Oberliga Nord and NOFV-Oberliga Süd.
The champions of each, BFC Dynamo and 1.
FC Magdeburg, entered into a play-off against each other for the right to play in the 2001–02 Regionalliga Nord.
FC Magdeburg won 5–2 over two legs and thus gained promotion.