The 2000–01 Segunda Divisão season was the 67th season of the competition and the 51st season of recognised third-tier football in Portugal.
[1] The league was contested by 59 teams in 3 divisions with UD Oliveirense, Moreirense FC and Portimonense SC winning the respective divisional competitions and gaining promotion to the Liga de Honra.
The overall championship was won by Moreirense FC.
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