The 2000 Cal Poly Mustangs football team represented California Polytechnic State University, San Luis Obispo as an independent during the 2000 NCAA Division I-AA football season.
Led by Larry Welsh in his fourth and final season as head coach, Cal Poly compiled a record of 3–8 for the third consecutive year.
Welsh finished his four-year tenure as head coach with a record of 19–25, for a .431 winning percentage.
[4] No Cal Poly Mustang players were selected in the 2001 NFL draft.
[5][6][7] The following finished their college career in 2000, were not drafted, but played in the NFL.