The 2000 Jacksonville State Gamecocks football team represented Jacksonville State University as a member of the Southland Football League during the 2000 NCAA Division I-AA football season.
Led by First-year head coach Jack Crowe, the Gamecocks compiled an overall record of 4–6 with a mark of 2–5 in conference play, placing sixth in the Southland.
Jacksonville State played home games at Paul Snow Stadium in Jacksonville, Alabama.
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