The 2000 NCAA Division II women's basketball tournament was the 19th annual tournament hosted by the NCAA to determine the national champion of Division II women's collegiate basketball in the United States.

[1] Northern Kentucky defeated North Dakota State in the championship game, 71–62 (after one overtime), to claim the Norse's first NCAA Division II national title.

The championship rounds were contested in Pine Bluff, Arkansas.

Location: Heiges Field House Host: Shippensburg University of Pennsylvania Location: Regents Hall Host: Northern Kentucky University Location: Bison Sports Arena Host: North Dakota State University Location: Activities Center Host: College of Saint Rose Location: Walter Sillers Coliseum Host: Delta State University Location: Templeton Physical Education Center Host: Presbyterian College Location: White Auditorium Host: Emporia State University Location: Kellogg Gym Host: California State Polytechnic University, Pomona Location: Pine Bluff Convention Center Host: University of Arkansas at Pine Bluff