For 2000, Pitt looked to find a replacement at defensive back for NFL drafted Hank Poteat.
Without starting quarterback Michael Vick, Virginia Tech came back in the fourth quarter, scoring 10 points, to win the game 34–37.
[7] Pittsburgh would then host North Carolina, who would upset the Panthers 17–20 despite holding a 407–293 disadvantage in total offense.
The contest was the first football game played at the home field of Major League Baseball's Arizona Diamondbacks, Bank One Ballpark.
Pitt scored on their first possession by way of a 72-yard Antonio Bryant touchdown reception from quarterback John Turman.
Pittsburgh would then give up a 72-yard punt return for a touchdown by Iowa State's JaMain Billups, 20–34.
Next, Pitt's Nick Lotz would kick a 25-yard field goal to make it 23–34 with 11:08 left in the game.
Pitt's comeback drive would be ended by a Marc Timmons interception at Iowa State's 12-yard line.