2000 U.S. Open Cup

Amateur club Uruguay SC narrowly missed a major second-round upset, losing in overtime to the Tampa Bay Mutiny.

Mid Michigan came closest to duplicating its second-round upset, losing in a shootout to eventual runner-up Miami.

Two referees were used for all games from the second round through the semifinals as part of a FIFA experiment.

Home teams listed on top of bracket Note: Scorelines use the standard U.S. convention of placing the home team on the right-hand side of box scores.

Twelve MLS, nine A-League, seven D3 Pro League teams enter.