The 2000 U.S. Women's Open was the 55th U.S. Women's Open, held July 20–23 at the Merit Club in Libertyville, Illinois, a suburb north of Chicago and west of Waukegan.

Karrie Webb won the first of two consecutive U.S. Women's Opens, five strokes ahead of runners-up Cristie Kerr and Meg Mallon.

The purse was $2.75 million, an increase of over 57% from the previous year, with a winner's share of $500,000.

Webb also picked up an additional $250,000 bonus from the Nabisco Grand Slam Challenge for winning two majors in the same year.

[2] This championship was scheduled concurrently with The Open Championship in Scotland, where Tiger Woods won his fourth straight major title at St. Andrews to secure his historic Tiger Slam.