The 2000 WTA Madrid Open (also known as the Open de España Villa de Madrid or Trofeo Volkswagen for sponsorship reasons) was a professional women's tennis tournament played on outdoor clay courts at the Club de Campo Villa de Madrid in Madrid, Spain from 22–27 May 2000.

It was the fifth edition of the event on the WTA Tour.

It was classified as a Tier III event on the 2000 WTA Tour.

The following players received wildcards into the main draw: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: 1: Rankings are as of May 15, 2000.

The following pair received wildcards into the doubles main draw: The following pair received entry as alternates: Before the tournament During the tournament