The Football League play-offs for the 2000–01 season were held in May 2001, with the finals taking place at Millennium Stadium in Cardiff.
In the First Division, Bolton Wanderers, who are aiming to return to the top flight after nearly 3 seasons outside the top flight, finished 4 points behind second placed Blackburn Rovers, who in turn finished 10 points behind champions Fulham, who returned to the top flight for the first time since 1968.
Preston North End who are aiming to return to the top flight for the first time since 1961, finished in fourth place in the table.
West Bromwich Albion, who are also aiming to return to the top flight for the first time since 1986, finished 4 points behind Birmingham City and Preston North End in sixth place.
Preston North End 2–2 Birmingham City on aggregate.