The 2001 NAIA Division I women's basketball tournament was the tournament held by the NAIA to determine the national champion of women's college basketball among its Division I members in the United States and Canada for the 2000–01 basketball season.

Two-time defending champions Oklahoma City defeated Auburn Montgomery in the championship game, 69–52, to claim the Stars' fourth NAIA national title.

The tournament was played at the Oman Arena in Jackson, Tennessee.

[1] The tournament field remained fixed at thirty-two teams, with the top sixteen teams receiving seeds.

The tournament continued to utilize a simple single-elimination format.