The 2001 NCAA Division II men's basketball tournament was the 45th annual single-elimination tournament to determine the national champion of men's NCAA Division II college basketball in the United States.
Officially culminating the 2000–01 NCAA Division II men's basketball season, the tournament featured forty-eight teams from around the country.
The Elite Eight, national semifinals, and championship were played, for the first time, at the Centennial Garden in Bakersfield, California.
After losing in the previous year's final, Kentucky Wesleyan (31–3) defeated Washburn in the final, 72–63, to win their record eighth Division II national championship.
Cloud State University Location: Brayboy Gymnasium Host: Johnson C. Smith University Location: Lee Arena Host: Washburn University Location: Hornets Training Facility Host: Queens College, with support from the NBA Charlotte Hornets Location: Haggen Court at Sam Carver Gymnasium Host: Western Washington University Location: Bakersfield Centennial Garden Host: California State University, Bakersfield