2001 NCAA Division I women's soccer tournament

[1] Santa Clara defeated North Carolina in the final, 1–0, to win their first national title.

The tournament's leading scorer, with 5 goals and 4 assists, was Abby Wambach from Florida.

All Division I women's soccer programs were eligible to qualify for the tournament.

Just as before, the final two rounds, deemed the Women's College Cup, were played at a pre-determined neutral site.

All other rounds were played on campus sites at the home field of the higher-seeded team.