By-elections for Strakonice and Brno-City District Senate seats were held in the Czech Republic in October and November 2003.

Elections were won by Josef Kalbáč in Strakonice and by Karel Jarůšek in Brno-City.

[1] Elections were held after incumbent Senators were appointed Judges of Constitutional Court of the Czech Republic.

Candidates included football manager Karel Jarůšek, Brno Councillor Rostislav Slavotínek, Minister of Health Marie Součková, Daniel Borecký and Journalist Petr Cibulka.

Minister Součková was eliminated in the first round when she received only 7.5% of votes which she blamed on strong campaign against her.