Venus Williams was unsuccessful in her title defence, losing in the third round against Jelena Janković.

[4] Shenay Perry was the only American player to reach the fourth round; she was defeated 6–2, 6–0 by Elena Dementieva after losing the last ten games of the match.

Venus Williams' third round defeat by Jelena Janković of Serbia (on its first Grand Slam appearance as a newly established independent nation from the former Serbia and Montenegro) caused the earliest exit by a defending women's champion at Wimbledon since Steffi Graf lost in the first round in 1994 and meant that neither of the Williams sisters (Serena Williams withdrew due to injury) would be represented in a Wimbledon final for the first time since 1999.

[6] These championships were also the first to feature three Serbian players in the fourth round of any Grand Slam tournament: along with Janković, Ana Ivanovic[7] and Novak Djokovic[8] also reached the fourth round, the former losing to Amélie Mauresmo and the latter losing to Mario Ančić.

China's Li Na became the first player from her country to ever be seeded or reach the quarter-finals of a Grand Slam tournament.

She upset the recent French Open finalist Svetlana Kuznetsova in the third round[9] and followed it up with a win over World No.