The 2007 NCAA Division I lacrosse tournament was the 37th annual tournament hosted by the National Collegiate Athletic Association to determine the team champion of men's college lacrosse among its Division I programs, held at the end of the 2007 NCAA Division I men's lacrosse season.

Johns Hopkins won their ninth national title, defeating Duke in the final 12–11.

[4] Delaware, led by Alex Smith made it to the Final Four for the first time in school history.

Sixteen NCAA Division I college men's lacrosse teams met after having played their way through a regular season, and for some, a conference tournament.

No teams made their debut appearance in the Division I men's lacrosse tournament.