The 2007 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 2007 NCAA Division I FCS football season.

Led by fourth-year head Jay Thomas, the Colonels compiled an overall record of 6–5 with a mark of 3–4 in conference play, placing in a three-way tie for fourth in the Southland.

Nicholls State played home games at John L. Guidry Stadium in Thibodaux, Louisiana.

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