Justine Henin defeated Svetlana Kuznetsova in the final, 6–1, 6–3 to win the women's singles tennis title at the 2007 US Open.

Maria Sharapova was the defending champion, but was defeated by Agnieszka Radwańska in the third round.

1 and five-time major singles champion Martina Hingis, who retired in November 2007 (she would later return to the sport in doubles starting in 2013).

It was also the first US Open main draw appearance for future world No.

1 and Australian Open champion Caroline Wozniacki, who lost in the second round to Alizé Cornet.