The 2009–10 Florida State Seminoles women's basketball team represented Florida State University in the 2009–10 NCAA Division I women's basketball season.

The Seminoles were coached by Sue Semrau.

The Seminoles were a member of the Atlantic Coast Conference.

The Seminoles won their second consecutive regular season title and were selected as a three-seed in the NCAA tournament where they advanced to the semifinals for the first time in school history