The 2009 Bancolombia Open was a professional tennis tournament played on outdoor red clay courts.

It was part of the 2009 ATP Challenger Tour.

The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: Horacio Zeballos def.

Santiago González, 7–6(3), 6–0 Sebastián Prieto / Horacio Zeballos def.

Alexander Peya / Fernando Vicente, 4–6, 6–1, [11–9]