The 2009 Copa do Brasil de Futebol Feminino was the third staging of the competition.

[1] 32 clubs of all regions of Brazil participated of the cup, which is organized by the Brazilian Football Confederation (CBF).

[2] The winner of the cup represented Brazil in the 2010 Copa Libertadores de Fútbol Femenino.

[3] The competition was contested by 32 clubs in a knock-out format where in the first three rounds was played over two legs and the away goals rule was used, but if the away team won the first leg with an advantage of at least three goals, the second leg would not be played and the club automatically qualified to the next round.

[2] The final and the third-place game was played in one leg in a neutral venue.