Head coach Jim Haslett left the team in January 2010, becoming the new defensive coordinator for the Washington Redskins.

[1] On February 9, 2010, Jay Gruden was named the team's new head coach and general manager by UFL commissioner Michael Huyghue.

On the same day of Gruden's appointment, the Tampa Bay Rays were announced to have sold their interest in the franchise, which meant that all home games for the Tuskers would be played at the Citrus Bowl in Orlando.

[2] Along with the league's other teams, the Tuskers unveiled new uniforms during an event at Fashion Show Mall in Las Vegas on July 28, 2010.

Rookies in italics Roster updated November 18, 2010 52 Active, 10 Inactive y-denotes team has clinched a 2010 UFL championship game berth x-denotes team has been eliminated from championship contention at Sam Boyd Stadium, Whitney, Nevada