On Thursday, reigning world champion Jorge Lorenzo crashed heavily during free practice in wet conditions and fractured his left collarbone as a result.
The second row of the grid consists out of Valentino Rossi in fourth, Dani Pedrosa in fifth and Bradley Smith in sixth place.
Iván Silva replaces Hiroshi Aoyama who is still recovering from a crash in free practice on the Saturday of the Catalan round, losing a part of his finger as a result.
Further back, Nicky Hayden gets passed by Aleix Espargaró who takes a tighter line at Ossebroeken (Turn 4) for seventh spot.
Lorenzo then uses the opportunity to quickly move past the American on the outside of Strubben as well for eighth place on the opening lap.
At the entrance of the Geert Timmer Bocht (Turn 16), Rossi dives down the inside of Bradl and manages to take third.
On lap two, Lorenzo makes a lunge up the inside of Smith at the end of start/finish straight to pass the Briton for sixth position entering the Haarbocht.
At the run up to Duikersloot (Turn 11), Crutchlow goes a bit wide and Lorenzo makes good use of this by taking a shorter line a passing the Briton for fourth position.
Lap five and Rossi dives down the inside of Márquez entering Haarbocht, taking second from him as the fans in the main grandstands cheer him on.
Rossi is now opening a gap in pursuit of Pedrosa, Lorenzo doing likewise on Crutchlow to chase down Márquez.
At the Geert Timmer Bocht, Rossi goes up the inside of Pedrosa and takes over the lead of the race at the exit.
At the entrance to De Bult, Márquez has a moment trying to stop his bike but doesn't lose any significant time over it.
Lap nine and Pedrosa is still relatively close to Rossi in second as third Márquez now starts to come under pressure from Lorenzo in fourth.
Lap thirteen - the halfway point of the race - and Márquez is closing the gap to teammate Pedrosa.
Crutchlow is still right behind Lorenzo and attempts a move at Strubben but misses the speed to get past as the Spaniard also closes the door on him.
At the Geert Timmer Bocht, Crutchlow finally manages to get past by diving down the inside of Lorenzo at the entrance of the chicane, taking fourth as a result.
At the end of the Veenslang, Márquez carries a lot more speed at the new, quick right-hand kink but runs wide entering the Ruskenhoek, allowing Pedrosa to get back at him exiting the turn.
At the Geert Timmer Bocht, Márquez makes his move by going up the inside of his teammate and taking second place.
Lap nineteen and Rossi's gap back to Márquez is +1.044 seconds, showing that he has increased it since the pair were fighting.
Entering Strubben, Crutchlow dives too deep and goes wide, giving some breathing room to Pedrosa.
Entering the Haarbocht, Crutchlow makes a mistake and almost runs into Márquez but just manages to avoid him, ending his hopes for a second place.
Fourth is Pedrosa and Lorenzo comes home a superb fifth after struggling with a broken left collarbone only 36 hours earlier.
On the parade lap back to parc-fermé, Rossi waves gleefully at the fans after winning a race in nearly three years.
As Lorenzo arrives at the pits, he is seen visibly in pain and even cries as he needs help from his team to push the bike back inside.
Rossi - still riding back - briefly waves at the crowd, then puts his arm up in the air multiple times.
By now, Márquez takes off his helmet as Rossi finally enters the pits, still with his arm up in the air as a salute to his fans.
A happy Márquez talks and shakes hands with an equally delighted Crutchlow as Rossi finally arrives at parc-fermé, steps off his bike and immediately goes to celebrate with his team.