The Cup draw was held on 12 February 2014 in Tashkent.
The competition started on 1 April 2015, and will end in November 2015 with the final to be held at the Pakhtakor Markaziy Stadium in Tashkent.
The cup winner is guaranteed a place in the 2016 AFC Champions League.
The sixteen winners from the Round of 32 were drawn into eight two-legged ties.
The second legs matches Pakhtakor - Semetchi Kuvasoy and Bunyodkor - Sogdiana Jizzakh will be played on 28 July 2015.