The 2016 Brasil Open was a tennis tournament played on outdoor clay courts.

It was the 16th edition of the Brasil Open, and part of the ATP World Tour 250 series of the 2016 ATP World Tour.

It took place from February 22 through February 28, 2016, in São Paulo, Brazil.

Third-seeded Pablo Cuevas won the singles title.

The following players received wildcards into the main draw: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: The following pairs received wildcards into the main draw: The following pair received entry as alternates: