The defending champions, Leeds Rhinos who were looking to win the trophy three times in a row were defeated by Huddersfield Giants in the sixth round.

These are the four teams that finished in the bottom four positions of the 2015 Super League and are; Widnes Vikings, Hull Kingston Rovers, Salford Red Devils, and Wakefield Trinity Wildcats.

The third round draw was held on 16 February at North Wales Crusaders ground, the Glyndwr University Racecourse Stadium, Wrexham.

This round sees the first entry of Super League clubs when the teams that finished 9th – 12th in the 2015 season are in the draw.

[10] The draw for the sixth round was made on 21 April 2016 live on BBC Radio 4 Today programme.

The teams were drawn by Today presenter John Humphrys and former England and Great Britain international player Martin Offiah.

[13] The quarter final draw was made after the conclusion of the St Helens vs Hull game, live on BBC 2.

The draw was made by Castleford and Leigh player Bob Beardmore and Ian Gildart who played for Wigan, Wakefield Trinity and Oldham.

[18] Teams: Hull: Jamie Shaul, Stevie Michaels, Mahe Fonua, Kirk Yeaman, Fetuli Talanoa, Carlos Tuimavave, Marc Sneyd, Scott Taylor, Danny Houghton, Liam Watts, Sika Manu, Mark Minichiello, Gareth Ellis (captain).

[18] Warrington Wolves: Stefan Ratchford, Matty Russell, Toby King, Ryan Atkins, Rhys Evans, Kurt Gidley, Chris Sandow, Chris Hill (captain), Daryl Clark, Ashton Sims, Ben Currie, Jack Hughes, Joe Westerman.