The 2016 Denver Stampede season was the first and only season in the club's history.

Coached by Sean O'Leary and captained by Pedrie Wannenburg, Denver competed in the United States' 2016 PRO Rugby competition which they won.

In 2016 Denver jerseys were made by Champion System.

All home matches were played at Infinity Park in Denver through May and then at the University of Denver's CIBER Field.