The women's 400 metres at the 2016 European Athletics Championships took place at the Olympic Stadium on 6, 7, and 8 July.

[1] All times are local times (UTC+2) First 3 in each heat (Q) and the next fastest 4 (q) advance to the Semifinals.

[2] First 2 (Q) and next 2 fastest (q) qualify for the final.

[3] *Athletes who received a bye to the semifinals[4] [5]