The women's 400 metres hurdles at the 2016 European Athletics Championships took place at the Olympic Stadium on 8, 9 and 10 July.

[1] All times are local times (UTC+2) First 2 in each heat (Q) and the next fastest 6 (q) advance to the Semifinals.

[2] First 2 in each heat (Q) and the next fastest 2 (q) advance to the Final.

[3] *Athletes who received a bye to the semifinals[4] [5]