The Women's 100 metre breaststroke competition of the 2016 FINA World Swimming Championships (25 m) was held on 9 and 10 December 2016.

[1] Prior to the competition, the existing world and championship records were as follows.

The heats were held at 11:00.

[2] The semifinals were held at 20:09.

[3] The final was held at 20:06.