The Women's 50 metre breaststroke competition of the 2016 FINA World Swimming Championships (25 m) was held on 6 and 7 December 2016.

[1] Prior to the competition, the existing world and championship records were as follows.

The heats were held at 11:12.

[2] The semifinals were held at 18:56.

[3] The final was held at 19:11.