The 2016 League 1, known as the Kingstone Press League 1 for sponsorship reasons, was a semi-professional rugby league football competition played in England, the third tier of the sport in the country.

The 2016 League 1 season was expanded to 15 teams with Toulouse Olympique rejoining the British game.

At the end of the weekly rounds the top 8 teams played each other once more in the League 1 Super 8s after which the top 5 teams competed in the play-offs.

The bottom 7 teams played each other once more with the top two teams competing for the inaugural League 1 Shield.

(Q) = Qualified for Play-offs (S) = Secured spot in 2017 Championship (Q) = Qualified for Play-offs Promotion Final Playoffs