The 2016 NCAA Division I Women's Lacrosse Championship was the 35th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse.
The semifinal and championship rounds were played at Talen Energy Stadium (the home of Major League Soccer's Philadelphia Union) in Chester, Pennsylvania from May 27–29, 2016.
[1] All other rounds were played at campus sites, usually at the home field of the higher-seeded team, from May 13–22.
All NCAA Division I women's lacrosse programs were eligible for this championship, and a total of 26 teams were invited to participate.
13 teams qualified automatically by winning their conference tournaments while the remaining 13 teams qualified at-large based on their regular season records.