The second edition of the women's Strade Bianche was held on 5 March 2016, in Tuscany, Italy.

British world champion Lizzie Armitstead won the race, in bad weather, ahead of Katarzyna Niewiadoma and Emma Johansson.

[2][3] The race is organized on the same day as the men's event, at a shorter distance, but on much of the same roads.

[3] The Strade Bianche is a one day cycling race starting in and finishing in Siena,[3] notorious for its long sections of white gravel roads (sterrati or strade bianche in Italian).

[5] The race finished on Siena's Piazza del Campo, after a narrow ascent on the roughly-paved Via Santa Caterina in the heart of the medieval city.