The 2016 Lamar Hunt U.S. Open Cup was the 103rd edition of the oldest ongoing competition in American soccer.
[1] This rule prevented several United Soccer League teams (Bethlehem Steel FC, LA Galaxy II, New York Red Bulls II, Orlando City B, Portland Timbers 2, Real Monarchs, Seattle Sounders FC 2 and Swope Park Rangers) from competing.
Major League Soccer (MLS) teams New York Red Bulls, Portland Timbers, and Seattle Sounders FC each entered their under-23 amateur sides.
This rule prevented United Soccer League teams Bethlehem Steel FC, LA Galaxy II, New York Red Bulls II, Orlando City B, Portland Timbers 2, Real Monarchs, Seattle Sounders FC 2 and Swope Park Rangers from competing.
Host team listed firstBold = winner* = after extra time, ( ) = penalty shootout score Draw announced April 6.
The draw to determine the semifinal hosts for the final teams from the eastern and western halves of the bracket took place on July 21 at Soccer House in Chicago and was conducted by U.S. Open Cup Commissioner Paul Marstaller.