The Women's time trial of the 2016 UCI Road World Championships took place in and around in Doha, Qatar on 11 October 2016.

Linda Villumsen won her first world time trial title in 2015 but did not take part in the 2016 race.

2008 world champion Amber Neben, from the United States, claimed her second rainbow jersey,[2] completing the course 5.99 seconds quicker than the European champion Ellen van Dijk, from the Netherlands.

Australia's Katrin Garfoot, the Oceanian champion, won the bronze medal, 2.33 seconds behind van Dijk and 8.32 seconds in arrears of Neben's winning time.

[6][7] [1] * Aljersiwi fell during her race by riding into a fence.