[10][11] On June 13, 2016, despite his previous statements that he would not run for re-election to his Senate seat, Rubio "seemed to open the door to running for re-election," citing the previous day's mass shooting in Orlando and how "it really gives you pause, to think a little bit about your service to your country and where you can be most useful to your country.

Rubio won with the largest raw vote total in Florida history (until Donald Trump broke the record in 2020), taking a greater percentage of the popular vote than Republican presidential nominee Donald Trump, who won the state in the election.

Representatives Statewide officials State legislators Mayors and other municipal leaders Labor unions Organizations Newspaper Editorial Boards On October 1, 2015, Adrian Wyllie and Lynn House, chair and vice chair, respectively, of the Libertarian Party of Florida, resigned their seats in protest after the executive committee refused to oust candidate Augustus Invictus from the party.

According to Wyllie, Invictus had defended eugenics, called for a new Civil War, and brutally slaughtered a goat, and is not representative of the Libertarian Party.

"[233] Individuals Libertarian Party of Florida Affiliates Organizations Individuals Declared Graphical summary with Ron DeSantis with David Jolly with Carlos Lopez-Cantera with Jeff Atwater with Pam Bondi with Don Gaetz with Jeff Miller with Marco Rubio with Allen West with Todd Wilcox with Carlos Beruff with Alan Grayson Rubio won 16 of 27 congressional districts, with the remaining 11 going to Murphy.