Northampton Saints entered the competition as reigning champions, becoming the first winners of the Premiership Cup when they defeated Saracens 23 – 9 in the final at Franklin's Gardens during the 2019–20 season.

The winners of the semi-finals then met in the final delayed from March to September 2020, held at the home ground of the highest ranked remaining team.

[4] The pool stage began on 20 September 2019 and ran for four consecutive weeks.

Semi-finals were held over a two-week period in early February followed by the final originally scheduled for mid-March but postponed to September due to the COVID-19 pandemic.

57 – 23 Worcester Warriors at home to Leicester Tigers on 21 September 2019 49 – 22 Harlequins away to Exeter Chiefs on 31 January 2020 57 – 23 Worcester Warriors at home to Leicester Tigers on 21 September 2019 Worcester Warriors at home to Leicester Tigers on 21 September 2019 Worcester Warriors at home to Leicester Tigers on 21 September 2019 Exeter Chiefs at home to Bristol Bears on 12 October 2019 Saracens away to Wasps on 21 September 2019 Leicester Tigers away to Bath on 5 October 2019